Establishment and Efficacy of an Endoscopic Pathogen Visualization Literacy (EPVL) Training Program for Gastroenterologists Based on Fluorescence Rapid On-Site Evaluation (ROSE) Technology

<jats:p>Objective: To establish a standardized training program for endoscopic pathogen visualization literacy (EPVL) based on fluorescence rapid on-site evaluation (ROSE) technology for gastroenterologists, and to evaluate its training efficacy. Methods: A prospective quasi-experimental study was conducted. A total of 54 gastroenterology trainees were non-randomly allocated into the EPVL training group (Group A, n=28, 16-hour comprehensive training) and the control group (Group B, n=26, 3.5-hour traditional teaching). Pre- and post-training assessments included theoretical examinations, fluorescence ROSE image interpretation tests (30 parallel images per set), interpretation speed measurement, and clinical decision-making integration evaluation. The primary outcome was the change in image interpretation accuracy, analyzed by ANCOVA with pre-test scores as the covariate. Results: Baseline characteristics were comparable between groups (P greater than 0.05 for all demographic variables and pre-test scores). Group A showed significant improvement in image interpretation accuracy from 57.8 percent (SD 13.6) pre-training to 82.5 percent (SD 11.2) post-training (improvement of 24.7 percent, paired t = -12.86, P less than 0.001), while Group B improved from 58.5 percent (SD 13.0) to 71.0 percent (SD 13.5) (improvement of 12.5 percent, paired t = -5.24, P less than 0.001). After ANCOVA adjustment for pre-test scores, the between-group difference was significant (F(1, 51) = 10.95, P = 0.0017, eta squared = 0.177), with Cohen's d = 0.94 (large effect size). Interpretation speed in Group A (19.2 seconds per image, SD 2.8) was significantly faster than in Group B (32.5 seconds per image, SD 6.0, t = -10.45, P less than 0.001). Clinical decision-making scores were significantly higher in Group A (80.5, SD 8.0 vs. 65.3, SD 11.5, t = 5.60, P less than 0.001). The Kappa agreement with the gold standard in Group A improved from 0.56 (SD 0.18) to 0.84 (SD 0.11, t = -8.35, P less than 0.001). Participant satisfaction exceeded 88 percent. Conclusion: The EPVL training program significantly improves gastroenterologists' fluorescence ROSE image interpretation accuracy, speed, and clinical decision-making integration, providing a novel and effective standardized training paradigm for digestive endoscopy education.</jats:p>