MG2Act: A Mechanism-Inspired Sequential Attention Framework for Molecular Glue Degradation Prediction

<jats:title>Abstract</jats:title> <jats:p>Molecular glue degraders act by inducing productive proximity between an E3 ligase and a substrate protein. For most characterized degradative glues, a small molecule first engages the E3, conditions its substrate-recognition surface, and only then enables recruitment of a compatible neo-substrate. This directionality is rarely encoded explicitly in computational models, which typically fuse molecule, E3 and target representations simultaneously. We present MG2Act, a structure-independent framework that translates this two-step logic into sequential cross-attention, using CRBN-mediated degradation as the most data-rich representative system. Starting from a curated continuous-valued benchmark of 1,207 pairs across 47 targets, a refined subset of 1,159 pairs was selected to train MG2Act after excluding rare targets. On identical processed data, MG2Act consistently outperforms machine learning baselines, robustly generalizes under strict redundancy-filtering, and responds coherently to mechanism-based perturbations. Prospective screening and zero-shot target-conditioned prioritization identified nanomolar degraders of IKZF1, CK1α and CDK4, including the non-classical IMiD-core CDK4 degrader SWC-202.</jats:p>