Abstract
<jats:p>Background: Resveratrol is a naturally occurring polyphenolic stilbene widely distributed in grapes, berries, peanuts, and several medicinal plants. It has attracted considerable scientific interest because of its antioxidant, anti-inflammatory, cardioprotective, neuroprotective, anticancer, and antidiabetic activities. Reliable analytical methods are essential for the quantitative estimation of resveratrol in biological samples during pharmaceutical research and laboratory investigations. Objective: The present work aimed to develop a simple, economical, and rapid UV spectrophotometric method employing a protein precipitation procedure for the estimation of resveratrol in biological fluid and to evaluate its linear analytical performance. Methods: Biological fluid samples were subjected to protein precipitation using a methanol–acetonitrile (1:1, v/v) solvent system. After centrifugation, the clear supernatant was diluted appropriately with methanol and analysed using a Shimadzu UV–Visible spectrophotometer. The analytical wavelength was selected after recording the absorption spectrum of resveratrol over the ultraviolet region. Calibration standards were prepared over the concentration range of 2–10 µg/mL. Results: The optimized extraction procedure produced a clear supernatant with minimal matrix interference, allowing direct UV analysis. Resveratrol exhibited maximum absorbance at 290 nm. The calibration curve demonstrated excellent linearity over the investigated concentration range with a correlation coefficient (R2) of 0.999, indicating a strong linear relationship between concentration and absorbance. Conclusion: The proposed method provides a rapid, straightforward, and cost-effective analytical procedure for the estimation of resveratrol in biological fluid. Owing to its simplicity and minimal sample preparation requirements, the method may be useful for routine laboratory investigations, educational research, extraction studies, and preliminary bioanalytical applications.</jats:p>