Abstract
<title>Abstract</title> <p>MSCs-derived exosomes and placental peptide-based drug preparations are considered potential cell-free therapies due to their antioxidant and regenerative abilities. Antioxidant ability of peptides extracted from MO Placenta and exosomes obtained from MSC was assessed on ADF cell model under oxidative stress condition induced by H₂O₂. Exosomes were isolated from MSC conditioned media and characterized via TEM analysis. MO Placenta peptides and MSC-derived exosomes efficiently inhibited the H₂O₂-induced oxidative stress in ADF cells, which resulted from decreased ROS production and protein oxidation, as well as from the maintenance of intracellular antioxidant mechanisms. Treatment of cells with MO Placenta peptides resulted in an increase in the content of cellular thiols and antioxidant capacity. They also promoted migration of fibroblast under H₂O₂-induced oxidative stress condition. In the case of HUVECs, both of the peptides and exosomes significantly increased endothelial tube formation. Angiogenic ability of higher concentration of exosome was higher compared to the lower concentration and the 20 µg/mL peptide demonstrated more pro-angiogenic properties than 4 µg/mL peptide. The peptides obtained from MO Placenta and exosomes secreted by MSCs can effectively prevent the oxidative stress in adult dermal fibroblasts and boost angiogenesis of endothelial cells.</p>