Abstract
<jats:p>Patients receiving Methotrexate (MTX) treatments commonly encounter numerous side effects. Zinc oxide nanoparticles have gained attention in various fields, including medicine, due to their potential applications in drug delivery, imaging and therapy. This investigation aimed to assess the effects of Zinc oxide nanoparticles on male mice exposed to Methotrexate via isozyme electrophoresis and Semi-Quantitative RT-PCR. Polyacrylamide electrophoresis was utilized to separate different isozyme systems, such as Malate dehydrogenase, Esterase, Superoxide dismutase, and Lactate dehydrogenase, in order to identify specific biochemical tags associated with the effects of Methotrexate (MTX) and/or Zinc Oxide nanoparticles (ZnONPs). The results showed that Zinc oxide nanoparticles and/or MTX can alter the intensity and/or the numbers of some isozyme bands in certain mice body organs. Isozyme systems have proven to be real tags of differential gene expression in some mice body organs (liver, kidney and spleen). The results showed Up-Regulation of the liver Sod gene expression under the experimental conditions. Further biological investigations are necessary to thoroughly evaluate the effects of Methotrexate (MTX) medications and effectively mitigate any potential side effects.</jats:p>