Supplementary Figure S7 from Reprogramming of TLR–Ferroptosis Signaling and Immunometabolic Pathways Overcomes Myeloid Suppression to Improve Checkpoint Blockade in Prostate Cancer

<jats:p>&lt;p&gt;Non-targeted C’ dots induce tumor-specific inflammatory responses in the Hi-Myc prostate cancer model. Ex vivo gene expression profiling of (a) tumor and (b) spleen harvested from Hi-Myc mice 4 days after the final dose of vehicle or non-targeted particles. Specific classes of genes (i.e., iron/ferroptosis-related, DAMPs, antigen presentation, and immune-related) were profiled. Transcripts were normalized to Gapdh and represented as fold changes, with significant changes indicated by values above 2 (dashed lines). Gene expression changes represented by bar plots at (c) 24 h and (d) 96 h post-particle administration in Hi-Myc mice, shown as log2 fold change transcript alterations relative to saline; significance indicated by values &gt; 1 (solid lines).&lt;/p&gt;</jats:p>